A Handbook Of Biochemistry. Review Study Questions and Laboratory Manuals – A Clinical Approach

Content

Preface

Safety rules in the biochemistry laboratory

Seminar “Amino acids”: Proteinogenic amino acids

Seminar “Proteins І”: Structure and function of proteins

Seminar “Proteins ІІ”: Globular and fibrous proteins

Practical works: Proteins

Determination of the isoelectric point (pI) of casein

Determination of hemoglobin concentration by Drabkin’s reagent

Seminar “Enzymes I”: Enzymes as biocatalysts. Nomenclature and classification. izoenzymes. Water soluble vitamins as cofactors. Kinetics of enzyme action.

Seminar “Enzymes IІ”: Regulation of the enzyme activity. Reversible and irreversible inhibition of enzymes. Allosteric enzymes. Constitutive and inducible enzymes.

Practical works: Enzymes

Determination the absolute specificity of urease

Calculation of Km and Vmax by Lineweaver-Burke method

Seminar “Bioenergetics І”: Macroergic compounds. Biological oxidation. Oxidative phosphorilation. Structure of the respiratory chain

Seminar “Bioenergetics ІІ”: Short electron-transport chains. Free-radical oxidation and antioxidant defence

Practical works: Bioenergetics

Detection of cytochrome c oxidase and peroxidase in muscle tissue

Quantitative determination of total polyphenol content in plant extracts by Folin-Ciocalteu method

Seminar “Metabolism of carbohydrates І”: Digestion of carbohydrates. Transport of monosaccharides in cells. Glycolysis

Seminar “Metabolism of carbohydrates ІІ”: Degradation of pyruvate. Tricarboxylic acid cycle. Pentose phosphate Pathway

Seminar “Metabolism of carbohydrates ІII”: Gluconeogenesis. Glycogenesis. Glycogenolysis

Seminar “Metabolism of carbohydrates” ІV: Metabolism of mannose, fructose, galactose and lactose

Seminar “Metabolism of carbohydrates V”: Glycosaminoglycans. Glycoproteins

Seminar “Metabolism of carbohydrates VI”: Summary on carbohydrates metabolism

Practical works: Metabolism of carbohydrates

Alcoholic fermentation

Blood glucose curves of a healthy individual and a patient with type 1 diabetes

Determination of fructose in urine by Seliwanoff’s reagent

Seminar “Metabolism of lipids I”: Dietary lipiids digestion. Transport of exogenous lipids

Seminar “Metabolism of lipids II”: Lipolysis. Degradation of even and odd numbered fatty acids. Synthesis and use of ketone bodies

Seminar “Metabolism of lipids III”: Biosynthesis of fatty acids and triacylglycerols. Endogenous lipids in plasma

Seminar “Metabolism of lipids IV”: Biosynthesis of cholesterol, bile acids and bile salts. Cholesterol Transport in blood – role of HDL and LDL

Seminar “Metabolism of lipids V”: Complex lipids and eicosanoids

Practical works: Metabolism of lipids

Urine reagent strip test for ketone bodies

Quantative determination of triacylglycerols in serum

Quantative determination of total cholesterol in serum

Seminar “Metabolism of amino acids I”: Digestion of dietary proteins. Transamination of amino acids. Oxidative and nonoxidative deamination of amino acids

Practical work: Metabolism of amino acids

Quantitative determination of alanineaminotransferase (ALT) in serum

Seminar “Metabolism of porphyrins”: Heme biosynthesis and degradation. Porphyrias. Hyperbilirubinemias. Iron metabolism.

Practical work: Metabolism of porphyrins

Quantitative determination of total and direct bilirubin in serum

Seminar “Metabolism of nucleotides ІІ”: Biosynthesis and degradation of pyrimidine nucleotides. Biosynthesis of desoxyribonucleotides

Practical work: Metabolism of nucleotides

Determination of uric acid content in serum

Seminar “Nutrition and metabolism of xenobiotics”: Metabolism of xenobiotics and endobiotics. Nutrition

Seminar “Calcium metabolism”: hormones that regulate calcium and phosphate metabolism

Seminar “Intercellular signaling I”: General principles of intercellular signaling. Intracellular receptors. Receptors – ion channels

Seminar “Intercellular signaling II”: G-proteiN coupled receptors. With intrinsic enzimatic activity

Seminar “Molecular biology”: replication, transcription, translation. Cycle regulation. Oncogenes and tumor-suppressor genes. Regulation of gene expression

Recommended literature